physiology pathology mechanism / structure X non mammalian Heterologous and homologous expression of two plant pleiotropic drug resistance transporters as a tool for their functional characterization and their purification

Chronic oral treatment with ivermectin increases expression of genes involved in drug disposition in mice Mélanie Albérich, Cécile Menez, Anne Lespine INRA – UMR 1331, Université de Toulouse, INP, TOXALIM, F-31027 Toulouse, France Heterologous and homologous expression of two plant pleiotropic drug resistance transporters as a tool for their functional characterization and their purification Amandine Baijot, Justine Fourmeau and Marc Boutry Institut des Sciences de la Vie , University of Louvain, 1348 Louvain-la-Neuve, Belgium A Pleiotropic Drug Resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta Manuela Désirée Bienert, Stephanie Elise Gerlitz Siegmund, Anna Drozak, Tomasz Trombik, Alain Bultreys, Ian Thomas Baldwin, and Marc Boutry Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud, 4-15, 1348 Louvain-la-Neuve, Belgium. Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, 07745 Jena, Germany. Département Sciences du vivant, Centre Wallon de Recherches Agronomiques, Chaussée de Charleroi 234, 5030 Gembloux, Belgium. microRNA33 targets the ABCA1 pump and regulates cholesterol metabolism Laure-Alix Clerbaux, Isabelle Gérin, Guido T. Bommer. Laboratory for chelical physiology, de Duve Institute, Brussels, Belgium ABCB1 1199G>A genetic polymorphism influences tacrolimus intracellular accumulation in HEK293 and K562 recombinant cell lines. Géraldine Dessilly, Laure Elens, Nadtha Panin, Arnaud Capron, Jean-Baptiste Demoulin, Vincent Haufroid 2 Louvain centre for Toxicology and Applied pharmacology, Institut de recherche expérimentale et clinique, Université catholique de Louvain, Brussels, Belgium ; Department of Clinical Chemistry, Cliniques Universitaires Saint-Luc, Université catholique de Louvain De Duve Institute Brussels, Université catholique de Louvain, Belgium. Influence of the basal cellular level of glutathione in triggering MRP1-cells death Lauriane Dury, Attilio Di Pietro and Hélène Cortay IBCP, UMR 5086, BMSSI, 7 passage du Vercors 69007 Lyon, France. Role of P-glycoproteins in the installation of parasite nematodes in the host. Issouf M, Guegnard F, Koch C, Sauvé C, Neveu C and Kerboeuf D INRA, UR1282 Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France Identification of substrates of a plant pleiotropic drug resistance transporter, NtPDR1 from Nicotiana tabacum Julien Roland, Emmanuel Peeters, Joseph Nader and Marc Boutry Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud, 4-15, 1348 Louvain-la-Neuve, Belgium. Over-expression of plant pleiotropic drug resistance transporters for their enzymatic and structural characterization Frédéric Toussaint, Amandine Baijot and Marc Boutry Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud, 4-15, 1348 Louvain-la-Neuve, Belgium. Functional investigation of a multidrug transporter of Pseudomonas aeruginosa Alice Verchère, Isabelle Broutin & Martin Picard Laboratoire de Cristallographie et RMN Biologiques (CNRS UMR8015), Faculté de Pharmacie, Université Paris Descartes 4 Avenue de l’Observatoire, 75006 Paris, France. Transfer of the CFTR chloride channel by membrane‐derived microparticles as a therapeutic approach for cystic fibrosis. C. Vituret , G. Gonzalez , A. Di Pietro , M. Chanson , P. Boulanger , & S.S. Hong 1 1 Université Lyon I & INRA UMR‐754, 50, av. Tony Garnier, Lyon 2 Unité BM2SI, Université Lyon 1‐ CNRS UMR 5086, IBCP, 7, Passage du Vercors, Lyon 3 Laboratory of Clinical Investigation III, Faculty of Medicine, University of Geneva, FMR, 64, av. de la Roseraie, 1211 Geneva 4, Switzerland Further information for registration and abstract submission (http://www.facm.ucl.ac.be/ABC). 8 oral presentations by junior scientists will be selected from submitted abstracts. Selected speakers will receive a grant. SAVE YOUR FILE in .DOC FORMAT / FILENAME: ABC2012-NAMEOF1stTAUTHOR (no spaces !) Session chosen : physiology pathology mechanism/structure non mammalian Title: Chronic oral treatment with ivermectin increases expression of genes involved in drug disposition in mice Authors and affiliations: Mélanie Albérich, Cécile Menez, Anne Lespine 1 1 INRA – UMR 1331, Université de Toulouse, INP, TOXALIM, F-31027 Toulouse, France melanie.alberich@toulouse.inra.fr, Cecile.Menez@toulouse.inra.fr, Anne.Lespine@toulouse.inra.fr