Effect of plant growth regulators on callus multiplication and in vitro plant regeneration in Bacopa monnieri L

Current investigation deals with the tissue culture studies on Bacopa monnieri which has very high morphogenic potential. Its leaf explants respond very readily to treatment with auxins and cytokinins. Indefinite number of plantlets were regenerated from leaf margin, without intervening callus on Murashige & Skoog (MS) medium supplemented with indole-3acetic acid (IAA) and kinetin (KN). Green embryogenic callus with indefinite number of micro shoots were developed on naphthalene acetic acid (NAA) supplemented medium when 2,4-dichlorophenoxyacetic acid (2,4-D) grown stem-derived callus was sub-cultured. During present investigation, stem (internode, node) explants exhibited callus induction, whereas, leaf explant showed a tendency to regenerate shoot and/or roots. In vitro grown plantlets were transferred to polypots containing sterile cocopeat and then series of steps were carried out for acclimatization. Survival rate was 100% when acclimatized plantlets were transferred to soil. Micropropagated plantlets were without any morphological abnormalities.