Hybrid Sex Pheromones of the Hibiscus

The sex pheromone of the hibiscus flower borer Rehimena surusalis (Walker) 19 (Lepidoptera: Crambidae) was analyzed by gas chromatography with electroantennographic 20 detection (GC-EAD) and GC-mass spectrometry (GC-MS). Three EAD-active components 21 were found in crude pheromone gland extracts of calling females. GC-MS and GC analyses 22 using synthetic chemicals and derivatization of the extracts identified three components as 23 (10E,12Z)-hexadeca-10,12-dienal (E10,Z12-16:Ald,), (10E,12E)-hexadeca-10,12-dienyl 24 acetate (E10,Z12-16:OAc) and (3Z,6Z,9Z)-tricosa-3,6,9-triene (Z3,Z6,Z9-23:HC). In field 25 tests, male moths were remarkably attracted to a ternary blend of E10,Z12-16:Ald, E10,Z1226 16:OAc and Z3,Z6,Z9-23:HC at a ratio of 1:5:14, but single and binary blend of either 27 compound showed only weak or no attraction activity. 28