1 Identification of Food-grade Subtilisins as Gluten-degrading Enzymes to Treat Celiac Disease 1 2

Identification of Food-grade Subtilisins as Gluten-degrading Enzymes to Treat Celiac Disease 1 2 Guoxian Wei , Na Tian , Roland Siezen, Detlef Schuppan, Eva J. Helmerhorst * 3 4 1 Department of Molecular and Cell biology, Henry M. Goldman School of Dental Medicine, Boston, MA 5 02118; Bacterial Genomics Group, Center for Molecular and Biomolecular Informatics, Radboud 6 University Medical Centre, Nijmegen, the Netherlands, Division of Gastroenterology, Beth Israel 7 Deaconess Medical Center, Harvard Medical School, Boston, MA 02215; 4 Institute of Translational 8 Immunology and Research Center for Immunology (FZI), University Medical Center, Johannes9 Gutenberg-University, 55131 Mainz, Germany 10 11 Short title: Subtilisins for Celiac Disease 12 13