Urinary Tract Infection and Antibiotics Resistance Pattern among the Escherichia coli and Klebsiella spp . isolated from the patients of urinary tract infections at RIMS , Ranchi , Jharkhand , 834009

Introduction: Urinary tract infections (UTIs) are amongst the most common infections encountered in clinical practice. Urinary tract infection can be caused by gram negative bacteria such as Escherichia coli, Klebsiella species, Pseudomonas species, Proteus species, Enterobacter species and gram positive bacteria like Staphylococcus aureus, Enterococcus species and coagulase-negative Staphylococcus species. Escherichia coli is the most common organism isolated from the patients of UTI followed by Klebsiella species. Injudicious and irrational use of antibiotics and extended spectrum beta-lactamase enzyme (ESBL) production by Enterobacteriaceace family may be the main reason for emerging antibiotic resistance. Aims and Objectives: The prospective study was conducted to determine the bacteriology of UTI and antibiotic resistance pattern among Escherichia coli and Klebsiella spp. isolates of urinary samples at Rajendra Institute of Medical Sciences, (RIMS), Ranchi. Materials and Methods: A prospective study from May 2018 to October 2018 was performed after obtaining ethical clearance from Institutional Ethical Committee of Rajendra Institute of Medical Sciences, (RIMS), Ranchi. A total of 300 patients with UTI attended to the bacteriology section and organism identification, culture and sensitivity pattern was carried out as per CLSI standards. Results and discussion: Out of the 300 samples (165 females and 135 males), only 55.8% samples (n=167) showed positive results for urinary tract infections. The isolates were Escherichia coli (57.4%), Klebsiella spp. (19.17%), Staphylococus aureus (9.8%), Pseudomonas (7.3%), Coagulase-negative Staphylococci (3.9%) and Others (1.9%) respectively. It was also observed that the prevalence of gram negative baceteria was much higher than the gram positive bacteria. Conclusion: From this study it is concluded that the sensitivity of Enterobactriaceace group of organisms to known antibiotics are decreasing. Even the drugs like Imipenem, Nitrofurantoin and Ceftriaxone are also becoming resistant to Escherichia coli and Klebsiella spp. Escherichia coli isolates showed low resistance to Amikacin and Gentamicin, while Klebseilla isolates showed high resistance to these agents despite being sensitive for many years. -------------------------------------------------------------------------------------------------------------------------------------Date of Submission: 16-01-2019 Date of acceptance: 31-01-2019 -------------------------------------------------------------------------------------------------------------------------------------