A co-culture of M 1 and M 2 type macrophages with primary bronchial epithelial cells cultured at the air-liquid interface ( ALI-PBEC )

composed of ciliated cells, mucus producing cells, and Club cells. We have developed a co-culture system, using primary airway epithelial cells isolated from cadaveric bronchial rings and either mast cells derived from CD34-selected cord blood cells or eosinophils isolated by negative selection from peripheral blood. Using this system, we have demonstrated that a feed-forward system between epithelial cells and mast cells augments epithelial cell IL33 production.