The Single Nucleotide Polymorphism Study On The Shank3 And Nlgn3 Gene In Association With Autism In Wenzhou Children

Objective: To study the SHANK3 and NLGN3 Gene in Association with Autism in Wenzhou Children for its Single nucleotide polymorphism; Methods: It studied a Wenzhou sample of origin consisting of 100 unrelated patients with ASDs. 31 tagging SNPS within SHANK3 and NLGN3 gene with a minor allele frequency (MAF) greater than 5% in the population were selected to capture the majority of the common variations. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for SNP genotyping; Results: A significant genetic association between an intronic SNP rs9616915 and female ASDS was observed, with T-allele having an increased risk to develop to autism. Significant differences of allele frequencies were detected for 3SNPs in NLGN3 gene contrasted between cases and controls for male and female samples separately (male: rs11795613, rs4844285 and rs5981079, female: rs11795613, rs4844285 and rs7051529). Neither single SNP nor Haplotype in SHANK3 and NLGN3 was detected to show significant association with ASDS, even after gender stratification analysis. Conclusion: The SNPS with rs9616915 and rs13057681 of SHANK3 gene, the SNPS with rs11795613, rs4844285 and rs5981079 of NLGN3 gene were associated with Autism in Wenzhou Children.