Association of Interleukin-10 (-1082 A > G ,-819 C > T and-592 C > A ) gene polymorphism and its serum level with metabolic syndrome in north Indian subjects

Metabolic syndrome (MetS) is an inflammatory disorder and various cytokines play important role in tilting balance towards disease state. Interleukin-10 (IL-10) is an important anti-inflammatory cytokine, but its genetic polymorphisms and serum levels in Indian metabolic syndrome subjects are still unknown. Three IL-10 gene polymorphisms [-1082A>G (rs1800896), -819 C >T (rs1800872) and -592C >A (rs1800871)] were genotyped with PCR-RFLP in metabolic syndrome subjects (n=384) and age/sex matched control subjects (n= 386). Serum IL-10 was measured using ELISA. Serum IL-10 level was significantly low in MetS subject and significantly correlated with clinico-biochemical parameters of metabolic syndrome. Out of three investigated promoter polymorphisms, IL-10 -819 C >T and -592C >A were significantly associated with risk of metabolic syndrome. The mutant alleles -819T and -592A of IL-10 gene polymorphism were significantly higher in metabolic