MiR-1533 p suppresses atherosclerosis cells ’ migration via target regulation of ROCK 1

The anomalous migration of vessel smooth muscle cells (VSMCs) and so-caused atherosclerosis may result in a series of diseases including coronary heart disease (CHD). MicroRNAs (miRNAs) and their target genes play key roles in this complicated process. The function of microRNA-153-3p (miR-153-3p) in VSMCs migration remains unknown. In the present study, we found out that miR-153-3p was decreased in the human plasma from patients with atherosclerosis and in low and highatherosclerosis cells that were simulated by oxidized low density lipoprotein (OX-LDL) stimulation. Also we verified that Rho associated coiled-coil containing protein kinase 1 (ROCK1), a cytoskeleton-related protein, was increased in the aforementioned samples and cells. Furthermore, we confirmed that up-regulation of miR-153-3p could promote atherosclerosis cells migration and inhibit ROCK1 expression. Then, through a luciferase reporter assay, we verified that miR-153-3p could target bind to ROCK1 3’ untranslated region (3’UTR). Finally, an antisense experiment was executed to ultimately elucidate that miR-153-3p could promote migration via target regulating ROCK1 in atherosclerosis cells. In summary, the outcomes of the present study may reveal a new molecular in target treatment for atherosclerosis and its associated coronary artery disease (CAD).