Transient Lentiviral Vector Production in HEK 293 T Cells Using the BioFlo ® 320 Control Station with a BioBLU ® 5 p Single-Use Packed-Bed Vessel

Lentiviral vectors (LVs), which are especially applicable to gene therapy, are promising vector types for the clinical trials of such treatments. Current bottlenecks in the production of LVs are caused mainly by the disadvantages of classical two-dimensional culture forms. Switching to bioreactors can eliminate those disadvantages and offer the benefits of process automation, tight regulation of production conditions, and reduced labor input. This application note describes the first successful experimental setup to cultivate LVs in HEK 293T cells adherently grown on Fibra-Cel® disks in a BioBLU 5p Single-Use Vessel. Functional unconcentrated titers (TU) between 105 106 TU/mL and physical vector particles (VP) of 108 109 VP/ mL were achieved, with no significant difference in quality compared to production on conventional cell culture plates, demonstrating the great potential for LV production in stirred-tank bioreactors on Fibra-Cel disks.