A Novel Phenyl Bonded Phase for Improved Reversed-Phase Separations of Proteins

■ ■ Excellent acid stability for improved column lifetimes INTRODUCTION The inherent complexity and microheterogeneity of proteins requires that they be extensively characterized if they are to be used as therapeutic modalities, as is the case with many monoclonal antibodies (mAbs). High performance liquid chromatography is the most widely used technique for detecting the molecular variability of protein and peptide therapeutics. Reversed-phase liquid chromatography (RPLC) is particularly useful because of its high resolution and amenability to mass spectrometry detection. However, it can be a challenge to achieve efficient separations of proteins due to their size and complexity. RPLC separations of proteins are strongly dependent on the surface chemistry of the particle, where undesirable interactions with protein sites may cause peak broadening and high carryover.1