Selective Remove of lignin in Tobacco Stalk by Bacillus and its Laccase Producing Characteristic

In order to screen more microorganisms for the biodegradation of tobacco stalk and explore its application value in the reusing of tobacco stalk. One bacterium strain H02 was screened out from soil for detecting its selective degradation of lignin in tobacco stalk and relative higher laccase production when fermentation with tobacco stalk in liquid state. The identification of 16S rDNA sequence indicates that the bacterium is belonged to Bacillus sp. It showed the highest laccase enzyme activity of 332.41 U/mL at 10 d after fermentation and the removal ratio of lignin, cellulose and hemicellulose were 15.68%, 5.58% and 19.66% respectively. In shortly, it can be inferred that this may provided a new approach for the reusing of tobacco stalk.