DRUBE, Clairmont George, 1928- INTERRELATIONSHIP BETWEEN MIGRATION INHIBITION FACTOR AND TRANSFER FACTORS IN DELAYED-TYPE

In vivo and in vitro properties of the Transfer Factor (TF) and Migration Inhibition Factor (MIF) obtained from 2,4-dinitrofluorobenzene (2,4-DNFB) and tuberculin systems were studied simultaneously. Purified fractions of TF and MIF were obtained by Geon-Pevikon block electrophoresis and Sephadex G-200 exclusion chromatography. The physical and biological properties of these purified fractions were compared by in vivo and in vitro procedures. Transfer factors obtained from either the chemical or tuberculin system caused inhibi­ tion in the Migration Inhibition Factor-test (MIF-test) with normal guinea pig peritoneal exudate cells. When antigen was added to the crude transfer factors in the MIF-test there was an in­ creased inhibition of macrophage migration. Local cutaneous passive transfer of hypersensitivity in normal guinea pigs with TF and MIF from 2,4-DNFB system was unsuccessful, but local passive transfer sites of TF and MIF from the tuberculin system showed positive reactions. There was a distinctive difference in optical density patterns at 215 mp from Sephadex G-200 chromatographic fractionation of chemical and tuberculin migration inhibition factors. Analysis of the tuberculin system showed two 215 m// peaks whereas the Sephadex G-200 fraction­ ation of MIF from the chemical system resulted in only one 215 mfi peak which was equivalent to the second peak in the tuberculin system. Geon-Pevikon block electrophoretic fractionation of MIF did not resolve in vivo and in vitro biological activity into specific fractions. The biological activities extended from one fraction to another. The dialysis of crude transfer factor from chemical and tuberculin sensitive guinea pig peritoneal exudate cells resulted in a nondialyzable and a dialyzable TF. The dialysis of