Development of a specific isothermal nucleic acid amplification for the rapid and sensitive detection of shrimp allergens in processed food

Food Chemistry(2020)

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摘要
•A highly specific LAMP primer for the detection of shrimp DNA was developed.•The sensitivity of LAMP was better than that of conventional PCR for shrimp DNA detection.•Other Crustacea mixed with 0.01% shrimp did not interfere with the detection effectiveness of the LAMP assay.•Shrimp products were identified by LAMP in various processed commercial foods.•The developed LAMP assay can detect ingredients containing shrimp DNA and verify shrimp in commercial products.
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关键词
Food allergen,Shrimp,Loop-mediated isothermal amplification (LAMP),DNA detection
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