A retrospective analysis the clinic data and follow-up of non-invasive prenatal test in detection of fetal chromosomal aneuploidy in more than 40,000 cases in a single prenatal diagnosis center.

•We evaluate the detailed clinical follow-up information of 40,311 cfDNA screening cases in our prenatal diagnosis center.•The case of unconfirmed high-risk results, 44.28% directly to TOP, 41.4% had live births and 14.29% were lost to follow-up.•In the group of 39572 cases with low-risk results, 0.1% underwent invasive testing and confirmed six abnormal cases.•In cases with RAT and CNV results, 189 cases underwent invasive testing, revealing 5 cases RAT and 4 pathogenic CNVs.•Our study provided information that may help us with result interpretation, genetic counseling and the decision making in clinic.