Chaptalia Nutanspolak: Root Extract Has Highin Vitroantioxidant Activity And Low Cytotoxicityin Vivo

The Asteraceae family is widely known for its therapeutic, aromatic, and nutritional properties.Chaptalia nutans(C. nutans), a member of the family, is widely used in folk medicine in southern Brazil. In this study, we aim to assess compounds present in root extracts ofC. nutans, and evaluate their antioxidant capacity and toxicity. To determine the chemical composition of the extract, was performed through Liquid Chromatography coupled with Mass Spectroscopy. Antioxidant capacity, toxicity (Artemia salinabiosassay), cytotoxicity, genotoxicity (Allium cepatest), and neurotoxicity (Drosophila melanogastermodel) were evaluated. A large number of bioactive phytoconstituents were determined to be present, such as alkaloids, coumarins, flavonoids, terpenes, and especially phenolic compounds, which may explain the antioxidant capacity of the extract. Extracts had the capacity to protect cells from protein and lipid damage, and inhibit the formation of oxygen radicals. TheA. salinabioassay revealed that extracts were only slightly toxic. InA. cepa,cells exposed to 1.5 mg/mL extract were protected against chromosomal damage caused by glyphosate, and had mitotic index values that were reduced by 49%. A concentration of 10 mg/mL extract did not kill flies, and when coadministered with paraquat (PQ) (52.5%) produced a mortality rate of only 18.75%. These findings indicated that the extract had the potential to protect against PQ-induced neurotoxicity. Taken together, these data reveal for the first time that the root extract ofC. nutansis a rich source of natural antioxidants. The extract may be useful in the food and pharmaceutical industries.