Automated high content image analysis of dendritic arborization in primary mouse hippocampal and rat cortical neurons in culture.

•OmniBrain performs manual, semi- and fully automated Sholl analysis.•Manual cell selection and tracing increases inter-researcher variability.•OmniBrain eliminated researcher bias from neuron selection and image thresholding.•OmniBrain provides medium to high throughput analysis of dendritic morphology.