Insights on the participation of Glu256 and Asp204 in the oligomeric structure and cooperative effects of human arginase type I.

•Arginase has important biomedical functions in vascular diseases and asthma.•We analyzed new residues that participate in maintain the oligomeric state.•We analyzed residues relevant for cooperative properties.•Asp204 is relevant to maintain the oligomeric state of the enzyme.•Glu256 residue participate in cooperative communication between subunits.