Electroactive Cu2O nanoparticles and Ag nanoparticles driven ratiometric electrochemical aptasensor for prostate specific antigen detection

•Electrochemical ratiometric sensor is proposed for the detection of PSA.•Electroactive Ag nanodots are leached through resorcinol-formaldehyde surface.•Electroactive Cu2O NPs were designed to grow on rGO/Fe3O4 NSs.•Electroactive Ag NPs and Cu2O NPs are detection and reference tags.•Electrochemical sensor achieves the accurate detection of PSA.