Hemolysis of PM10 on RBCs in vitro:An indoor air study in a coal-burning lung cancer epidemic area

Epidemiological studies have suggested that inhalation exposure to indoor ambient air from coal-burning envi-ronments is causally associated with respiratory health risks.In order to explore the toxicological mechanisms behind the adverse health effects,the hemolytic activity of PM10 (particulate matter with an aerodynamic diam-eter of 10urn or less) samples collected from homes burning coal in the recognized China "cancer village"Xuanwei were evaluated and matched against their trace elemental contents.The results demonstrated that the hemolytic activity of indoor PM10 in coal-burning environments ranged from 4.28％ to 5.24％,with a clear pos-itive dose-response relationship.Although low dose samples exhibited a reduced hemolytic activity,PM10 could have a toxic effect upon people in a coal-burning indoor environment for extended time periods.The concentra-tions of analyzed trace elements in PM10 samples ranged from 6966 to 12,958 ppm.Among the analyzed ele-ments,Zn,Ti,Ni,Cu,Pb,Ba,Mn,Cr and V were found at higher concentrations and accounted for over 95％ of the total elements.The concentrations of total analyzed elements in the PM10 samples revealed a significant pos-itive correlation with PM10 hemolytic activity.Of the analyzed elements,Zn,Pb and Cs positively correlated with hemolysis,while Li,U and V negatively correlated with the hemolysis of human red blood cells (RBCs).Therefore,the heavy metal elements could be one of the main factors responsible for the hemolytic capacity of indoor PM1o in coal-buming environments.