Biotechnologically produced fucosylated oligosaccharides inhibit the binding of human noroviruses to their natural receptors.

•A new fermentation process for the production of complex oligosaccharides is described.•The process allows the production of highly pure lacto-N-fucopentaose I (LNFP I).•The LNFP I can be enzymatically converted into histo-blood group antigens (HBGAs).•LNFP I, HBGAs and simple oligosaccharides were tested in norovirus binding assays.•Fucosylated oligosaccharides inhibited norovirus binding to model trisaccharides and mucins.