Bar-HRM for identification of cryptic earthworm species

Gaspard Baudrin,Virginie Roy,Agnès Gigon,Lise Dupont

Pedobiologia（2020）

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摘要

•Development of a method to identify cryptic earthworm species without any sequencing step.•A method combining HRM with DNA barcoding allowed to identify species within the Allolobophora chlorotica species aggregate.•The Bar-HRM method could be easily adapted to any complex of earthworm species.

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关键词

Allolobophora chlorotica,Complex of species,High resolution melting analysis,Mitochondrial lineages,Species identification

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