A Novel Approach to Synthesize TiO 2 Nanoparticles: Biosynthesis by Using Streptomyces sp. HC1

A simple, yet effective and rapid approach for the synthesis of titanium dioxide nanoparticles (TiO 2 NPs) using Streptomyces sp. HC1 was developed. The in vitro activity of TiO 2 NPs was demonstrated against human pathogenic bacteria and fungi. The synthesized nanoparticles were characterized using various methods and instruments, such as Zetasizer, X-ray diffraction, Fourier transmission infrared spectroscopy, atomic force microscopy, and scanning electron microscopy. TiO 2 NPs were observed to be spherical in shape with size in the range of 30 to 70 nm. Antimicrobial activities of TiO 2 NPs against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 35218, Candida albicans , ATCC 10231, and Aspergillus niger ATCC 6275 were evaluated. It was observed that TiO 2 NPs showed higher antimicrobial activity against bacteria (12 mm) than against fungi. Moreover, TiO 2 NPs also showed potent antibiofilm activity against Pseudomonas aeruginosa ATCC 27853. Hence, the present study highlights the biological synthesis and characterization of TiO 2 NPs, and their applications in the field of biomedical sciences.