Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps

Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardarchaeota and from a yet unknown microorganism are expressed in Escherichia coli and mammalian cells, and ion transport assays and patch clamp analyses are used to demonstrate SzR as a novel type of light-driven inward H + pump. The mutation of a cytoplasmic glutamate inhibited inward H + transport, suggesting that it functions as a cytoplasmic H + acceptor. The function, trimeric structure, and H + transport mechanism of SzR are similar to that of xenorhodopsin (XeR), a light-driven inward H + pumping microbial rhodopsins, implying that they evolved convergently. The inward H + pump function of SzR provides new insight into the photobiological life cycle of the Asgardarchaeota.