Simultaneous speculation of 401 monomeric or homo-oligomeric subunit structures of human cellular proteins, mining the information in 1901 native 2D protein maps reconstructed from one nondenaturing 2DE gel.

•A method to speculate monomeric or homo-oligomeric protein structures was presented.•Digitized 2D maps of 1901 human cellular proteins were examined for the analysis.•Apparent mass of a protein divided by its calculated mass suggested monomer number.•Monomeric or homo-oligomeric quaternary structures of 401 proteins were speculated.