New joint analysis of electromyography spectrum and amplitude-based methods towards real-time muscular fatigue evaluation during a simulated surgical procedure: A pilot analysis on the statistical significance.

•Real-time analyses of muscle conditions allow anticipating the fatigue appearance.•A statistical analysis of JASA parameters allows to validate or not the relevance of the method to evaluate muscular fatigue.•Evolutions of the original JASA method provide information on muscle conditions all along an occupational task.