Glycomyces Albidus Sp. Nov., A Novel Actinobacterium Isolated From Rhizosphere Soil Of Wheat (Triticum Aestivum L.)

A novel actinobacterium, designated strain NEAU-7082(T), was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed the affiliation of strain NEAU-7082(T) to the genus Glycomyces. 165 rRNA gene sequence analysis indicated that strain NEAU-7082(T) belonged to the genus Glycomyces and was closely related to Glycomyces mayteni JCM 16217(T) (99.0% 16S rRNA gene sequence similarity), Glycomyces sambucus DSM 45047(T) (98.4%), Glycomyces scopariae DSM 44968(T) (98.3%), Glycomyces paridis DSM 102295(T) (98.1%), Glycomyces artemisiae NBRC 109773(T) (98.0%) and Glycomyces dulcitolivorans DSM 105121(T) (97.9%). Phylogenetic analysis using the 165 rRNA gene sequence showed that the strain formed a stable Glade with G. mayteni JCM 16217(T) and clustered with G. sambucus DSM 45047(T), G. scopariae DSM 44968(T). G. artemisiae NBRC 109773(T) and G. dulcitolivorans DSM 105121(T) in the genus Glycomyces. The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates were glucose and xylose. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG). glycolipid (GL), phosphatidylinositol mannoside (PIM) and an unidentified lipid (UL). The menaquinones were MK-11(H-4), MK-11 and MK-10. Major fatty acids were anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0. These chemotaxonomic data substantiated the affiliation of strain NEAU-7082(T) to the genus Glycomyces. The DNA G+C content was 71.3 mol%. A combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-7082(T) could be distinguished from its closest relatives. Therefore, strain NEAU7082(T) is considered to represent a novel species of the genus Glycomyces, for which the name Glycomyces albidus sp. nov. is proposed. The type strain is NEAU-7082(T) (=CCTCC AA 2019045(T) =JCM 33458(T)).