Microrna-500a-5p Inhibits Colorectal Cancer Cell Invasion And Epithelial-Mesenchymal Transition

The development of malignant tumors is a series of complex processes, the majority of which have not been elucidated. The aim of the present study was to investigate the microRNAs (miRNAs/miR) that affect the migration and invasion abilities of CRC cells. Our previous reports have revealed that miR-500a-5p suppressed CRC cell growth and malignant transformation. The present study demonstrated that overexpression of miR-500a-5p reduced the expression of vimentin, while increasing the expression of E-cadherin. Inhibition of miR-500a-5p resulted in spindle-like morphological changes and reorganization of F-actin in CRC cells. Furthermore, miR-500a-5p attenuated the transforming growth factor-beta signaling pathway in EMT. Additionally, emodin inhibited the miR-500a-5p inhibitor and suppressed the EMT process. In animal models of metastasis using nude mice, EMT and LoVo cell metastasis was modulated by miR-500a-5p. Therefore, the findings of the present study demonstrated that miR-500a-5p is associated with a positive therapeutic outcome in terms of invasion/migration of CRC cells and mesenchymal-like cell changes.