The complex genetic architecture of male mate choice evolution between Drosophila species

Mate choice behaviors are among the most important reproductive isolating barriers in many animals. Little is known about the genetic basis of reproductively isolating behaviors, but examples to date provide evidence that they can have a simple genetic basis. However, it is unclear if these results indicate that individual genes with large effects are common, or are instead due to ascertainment biases. Here, we present the results of a QTL mapping study for the most important behavioral isolating barrier between Drosophila simulans and D. sechellia: male mate choice. Our QTL results initially suggested that differences in male mate choice may be due to a couple loci with large effects. However, as we divided the largest-effect QTL using stable introgression strains, we found evidence of multiple interacting loci. We further find that separate regions of the genome control different aspects of male choice. Taken together, our results suggest that the genetic architecture of mate choice behavior, in this case, is more complex than QTL mapping suggested, highlighting potential challenges to future mapping studies. We discuss the implications of these results as they relate to signal–receiver coevolution, mate choice, and reproductive isolation.