A Nonhomologous End-Joining Mutant for Neurospora sitophila Research

Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: . Specifically, we show that deletion of increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC).