Combining quantitative and qualitative approaches using Sequential Window Acquisition of All Theoretical Fragment-Ion methodology for the detection of pharmaceuticals and related compounds in river fish extracted using a sample miniaturized method.

•A miniaturized method was validated for the analysis of 47 drugs in fish muscle.•Developed method: a fast ultrasound (USE) extraction and Z-Sep/C18 sorbent clean-up.•HPLC–QToF-MS data adquisition with recording SWATH mode.•Most frequently detected drugs: caffeine, carbamazepine, diltiazem and verapamil.•Suspect screening retrieved benzoylecgonine, cocaine, nicotine, and ofloxacin.