Evolutionary trends in bacteria isolated from moderate and severe diabetic foot infections in a Portuguese tertiary center.

•In our study, we found an increase in the isolation of gram-negative microorganisms in diabetic foot infections (DFI).•The Enterobacteriaceae family replaced Staphylococcus aureus as the most prevalent pathogen in DFI.•The incidence of neuroischemic diabetic foot ulcers (DFU) increased compared to purely neuropathic infected ulcers.•Chronic neuroischemic DFI usually present with distinct bacterial isolates.