A novel BSA immobilizing manner on modified titanium surface ameliorates osteoblast performance.

•Rutile phase titanium dioxide film was employed as titanium surface modifier.•The surface of TiO2 are mainly terminated by -OH groups which act as binding sites.•The oxide surface was functionalized with APTMS and BSA molecules.•APTMS molecule was used as spacer between the oxide and the BSA protein.•Biological analysis indicated better osteoblast performance.