Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis

•WT L-ASNase expressed in yeast was successfully glycosylated, secreted and purified.•The yeast strain was able to glycosylate all glycosylation sites of WT L-ASNase.•Mutations at L-ASNase glycosylation sites influenced its biological properties.•Mutated L-ASNase showed a significant increase in biological activity relative to WT.•Glycosylation increases L-ASNase stability and masks immunogenic epitopes.