Evaluation Of The Biocompatibility And Growth Inhibition Of Bacterial Biofilms By Zno, Fe3o4 And Zno@Fe(3)O(4 )Photocatalytic Magnetic Materials

Magnetic nanostructured materials have found numerous biomedical applications. However, the influence of a magnetic field on the inhibition of pathogenic microorganisms has been poorly explored. Zinc and Iron nano-structured oxides have been widely used due to their biocompatibility and their excellent optoelectronic and magnetic properties. Nevertheless, little effort has been devoted to demonstrate their antibacterial activity at doses that are not harmful to mammalians. In this work, ZnO, Fe3O4 (MNPs) and ZnO@Fe3O4 (NCs) were synthesized and fully characterized. The materials exhibit good antibacterial activity to inhibit the growth of Staphylococcus aureus (S. aureus) and Helicobacter pylori (H. pylori) both, as planktonic cells and as biofilms structures at low doses. The photocatalytic activity of the materials (NCs) was demonstrated when radiated suspensions of NCs and microorganisms (MOs) exhibited higher inhibition growth of MOs in comparison to non-radiated assays. The materials show better antibacterial activity for biofilm growth inhibition in comparison to commercially available antibiotics. Magnetic antimicrobial films were fabricated by in situ deposition of MNPs in Arabic gum (AG) solution. The films exert enhanced antibacterial activity against S. aureus growth due to Fe3+ lixiviation and magnetic disruption. Regarding the biocompatibility of the materials, ZnO modifies significantly biochemical parameters in Wistar rats after acute administration. Our results show that the composite ZnO@Fe3O4 at low doses: (a) exerts an optimum inhibition on the biofilm formation of microorganisms due to its synergetic activity of lixiviation of ions and oxidative activity; (b) good biocompatibility of the composite with living cells. These properties suit ZnO@Fe3O4 as potential candidates for the development of new anti-biofilm formulation.