Paracoccus Aurantiacus Sp. Nov., Isolated From Shallow-Sea Hydrothermal Systems Off Kueishantao Island

An orange-pigmented, short-rod-shaped, aerobic and non-motile bacterial strain, designated TK008(T). was isolated from the shallow-sea hydrothermal systems off Kueishantao Island in Taiwan. China, and it was studied by using a polyphasic taxonomic approach. Cells were Gram-stain-negative, and catalase- and oxidase-positive. Strain TK008(T) exhibited highest 16S rRNA gene sequence similarity of 97.1% to Paracoccus pacificus F14(T) . The phylogenetic trees based on 16S rRNA gene sequences showed that strain TK008(T) was a member of the genus Paracoccus. Digital DNA-DNA hybridization values between strain TK008(T) and two closely related species (Paracoccus zhejiangensis and Paracoccus tegillarcae) were 20.6 and 20.9%. The average nucleotide identity values of strain TK008(T) compared with P. zhejiangensis and P. tegillarcae were 75.2 and 74.6% respectively. The major isoprenoid quinone was ubiquinone-10. The predominant fatty acids (>10 %) were summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, two unidentified lipids and an unidentified glycolipid. The DNA G+C content of strain TK008(T) from genomic sequence data was 62.54mol%. On the basis of polyphasic analysis, strain TK008(T) represents a novel species, for which the name Paracoccus aurantiacus sp. nov. is proposed. The type strain is TK008(T )(=CGMCC 1.13898(T)=JCM 33630(T)).