Polyesterified Sesquiterpenoids From The Seeds Of Celastrus Paniculatus As Lifespan-Extending Agents For The Nematode Caenorhabditis Elegans

Eighteen new polyesterified dihydro-beta-agarofurans, celaspaculins A-R, as well as 11 known ones, were purified from the seeds of Celastrus paniculatus. The structures of new isolates were identified by extensive spectroscopic analysis and X-ray diffraction studies. The lifespan-extending activity of the dihydro-beta-agarofuran-enriched fraction and 10 abundant polyesterified dihydro-beta-agarofurans was evaluated on the nematode Caenorhabditis elegans model. Consequently, the dihydro-beta-agarofuran-enriched fraction and compounds 5, 14, and 21 notably increased the average survival time of C. elegans by 23%, 16%, 18%, and 17%, respectively, compared with the blank control group (p < 0.0001).