Simulation-Based optimisation of thermodynamic conditions in the esem for dynamical in-situ study of spherical polyelectrolyte complex particles in their native state.

•Mathematical-physical simulations of thermodynamics in the ESEM.•Gas flow, heat transfer and strength-stress simulations.•Monte carlo simulations of electron-gas, -water, -sample interaction and emission of signal electrons in the ESEM.•The most complex analysis of dynamic processes during in-situ observations in the ESEM.•Advanced observation of undamaged delicate wet spherical bio-particles in their native state.