Refining cell-based assay to detect MOG-IgG in patients with central nervous system inflammatory diseases.

•A full-length MOG transfected stable cell line and cell based assay was developed.•In-house and oxford CBA showed high concordance.•IgG (H + L) and igg1-Fc antibodies were comparable, and no IgM binding was observed.•CBA-immunofluorescence assay score and CBA-flow cytometry yielded high correlation.•No MS, AQP4-IgG positive NMOSD or healthy individuals were MOG-IgG seropositive.