Upregulation Of Circulating Mir-21 Is Associated With Poor Prognosis Of Nasopharyngeal Carcinoma

MicroRNAs (miRNAs) have been demonstrated to be implicated in the pathogenesis of a number of human diseases including cancer. The aim of current study was to reveal the potential clinical significance of serum miR-21 in nasopharyngeal carcinoma (NPC). CCK8 assay was used to evaluate the effects of miR-21 overexpression/downregulation on the chemoresistance of NPC cells. Real-time PCR was performed to detect the expression level of serum miR-21 in NPC patients and healthy volunteers. Then clinical significance of serum miR-21 was further investigated. Our results showed that miR-21 overexpression could enhance the chemoresistance of NPC cells to cisplatin, and vice versa. In addition, serum miR-21 was significantly upregulated in NPC patients and enhanced serum miR-21 level was associated with poor prognosis of NPC. Furthermore, its level was much higher in NPC patients resistant to cisplatin based chemotherapy and could discriminate the patients in the responding group from the non-responding group with high accuracy. The proportions of patients that resistant to chemotherapy were higher in the high serum miR-21 group. Finally, Kaplan-Meier survival analysis showed that enhanced serum miR-21 was a poor indicator of both overall and disease free survival among the patients who received cisplatin based chemotherapy. Taken together, serum miR-21 might be employed as a potential biomarker for predicting the clinical outcome and chemoresistance of NPC patients.