Pathway-based assessment of single chemicals and mixtures by a high-throughput transcriptomics approach.

•Concentration-dependent reduced transcriptomics for chemical and mixture assessment.•Specific pathways can be identified to quantitatively discriminate AHR agonists.•Single Chemicals were classified by integrating both potency and perturbed pathways.•Potency of mixture-induced pathways were predicable with an additivity assumption.