Serum Mir-10a-5p And Mir-196a-5p As Non-Invasive Biomarkers In Non-Small Cell Lung Cancer

Background: Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, accounting for about 85% of all cases. MicroRNAs are stable molecules in the blood and can be used as biomarkers for early diagnosis of various malignancies. The aim of this study was to evaluate expression of miR-10a-5p and miR-196a-5p in tissue and serum of patients with NSCLC and to explore its relationship with clinicopathological characteristics. Methods: A total of 20 pairs of tissues and 80 serum samples were obtained from NSCLC patients. Seventy-five serum samples from healthy individuals of the same age and gender were also collected. The expression level of miR-10a-5p and miR-196a-5p was detected by quantitative real-time PCR. The relationship between miR-10a-5p and miR-196a-5p expression level in NSCLC tissues and serum and clinicopathological characteristics was estimated respectively. The diagnostic value of miRNA-10a-5p and miR-196a-5p in NSCLC was assessed by the Receiver-operating characteristic (ROC) curve method. Results: We found that miRNA-10a-5p and miR-196a-5p expression levels were increased significantly in NSCLC tissues compared with non-tumor adjacent normal tissues. Serum miR-10a-5p and miR-196-5p were over-expressed in NSCLC patients compared with healthy controls. The higher miR-10a-5p or miR-196-5p expression levels were positively correlated with advanced tumor stage and positive lymph node metastasis. The area under the curve (AUC) of serum miR-10a-5p and miR-196-5p to diagnose NSCLC were 0.709 and 0.785. Optimal sensitivity and specificity were 65.98% and 72.71%, 67.86% and 77.57%, respectively in differentiating NSCLC patients from healthy controls. The combination of these two miRNAs with carcinoembryonic antigen (CEA) further increased the diagnostic value, with an area under the curve (AUC) of 0.801 (sensitivity, 76.34%; specificity, 79.26%) using logistic regression model analysis. Conclusions: Serum miR-10a-5p and miR-196a-5p may be useful noninvasive biomarkers for the clinical diagnosis of NSCLC.