Automated Co-Extraction of High-Quality DNA and RNA from Single Clinical FFPE Samples.

Formalin-fixed, paraffin-embedded (FFPE) preservation is the preferred method to archive clinical tissue biopsy samples for histopathological diagnosis. As advances in clinical molecular pathology continue to grow, the importance of reliable methods of extraction from FFPE tissue specimens become vital to ensure that patients receive timely and accurate reports. However, nucleic acid extraction from FFPE samples can be challenging and labor intensive, often resulting in degraded and fragmented DNA and RNA. Given the precious and limited nature of these clinical samples, the ability to differentially co-extract high-yield and high-quality DNA and RNA from a single sample input provides a tremendous advantage. Coupling the Covaris LE220R-plus Focused-ultrasonicator with liquid handling automation and the truXTRACFFPE kits for high-yield co-extraction, in this poster we demonstrates a standardized clinical FFPE extraction workflow providing downstream result confidence (higher yields and corresponding higher DV200 scores), increased efficiency, decreased sample variability, and reduction of manual touch points throughout the process. Furthermore, it is shown that the automated DNA and RNA workflows yield similar results as compared to manual methods using our truXTRAC FFPE kits.