Simulation and economic assessment of large-scale enzymatic N-acetyllactosamine manufacture

•Techno-economic analysis of large-scale enzymatic production of N-acetyllactosamine.•Separation processes based on chromatography and crystallization were compared.•The process based on selective crystallization and the BgaD-D enzyme gave the lowest costs.•Production capacity, N-acetylglucosamine recovery, acceptor to donor ratio and yield investigated.