An evaluation of glycated haemoglobin eluting in zone 10 on capillary zone electrophoresis

This is the first report of glycated haemoglobin (Hb) eluting in zone 10 on capillary zone electrophoresis (CZE). In an index case, the aberrant peak was picked up during routine haemoglobinopathy testing using CZE. Mass spectrometry (MS) revealed this aberrant peak to be glycated Hb. Prompted by this initial case, a study was undertaken and the results showed that there was a strong positive linear relationship (R-2 = 0.774; rho = 0.88) between the level of glycated Hb peak eluting in zone 10 and the HbA(1c) value. Furthermore, all samples with an HbA(1c) of 54 mmol/mol or greater had an HbA(1c) peak eluting in zone 10.