Antifungal mechanism of bacillomycin D from Bacillus velezensis HN-2 against Colletotrichum gloeosporioides Penz

Anthracnose is a leaf spot, blossom blight, or fruit rot disease caused by Colletotrichum gloeosporioides (Penz.). It is the most prevalent disease in mango-growing countries worldwide. Lipopeptides, such as those in the iturin family, account for the majority of antifungal secondary metabolites in Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus velezensis, and includes bacillomycin D. Thus far, the mechanism of bacillomycin D's activity has not been clear. In this study, bacillomycin D was isolated from B. velezensis HN-2, which strongly inhibits C. gloeosporioides (Penz.). The median inhibitory concentration of bacillomycin D was 2.162 μg/mL, causing deformation and damage to C. gloeosporioides (Penz.). Bacillomycin D showed more potent activity against C. gloeosporioides (Penz.) than two common fungicides prochloraz and mancozeb. Scanning and transmission electron microscopy revealed that bacillomycin D could injure the cell wall and cell membrane of the hyphae and spores of C. gloeosporioides (Penz.), and the cytoplasm and organelles inside the cell were exuded and formed empty holes. This research clarifies the mechanism underlying bacillomycin D antifungal activity and reveals its high potential as a biopesticide to control phytopathogens.