1 H NMR spectroscopy-based metabolic profiling of Ophiocordyceps sinensis and Cordyceps militaris in water-boiled and 50% ethanol-soaked extracts.

•Water and 50% ethanol extracts of O. sinensis and C. militaris were compared.•52 metabolites were identified and quantified from O. sinensis and C. militaris.•O. sinensis and C. militaris could be clearly discriminated by PCA and PLS-DA.•30% more metabolites could be extracted by water-boiled compare to 50% ethanol.