Evaluation Of Optiflow (Tm)-Ms/Ms For Bioanalysis Of Pharmaceutical Drugs And Metabolites

Aim: Microflow tandemmass spectrometry-based methods have been proposed as options to improve sensitivity and selectivity while improving sample utility and solvent consumption. Here, we evaluate a newly introduced microflow source, OptiFlow (TM), for quantitative performance. Results/methodology: We performed a comparison of the OptiFlow and IonDrive (TM) sources, respectively, on the same triple quadrupole mass spectrometer. The comparison used a neat cocktail of commercially available drugs and extracted plasma samples monitoring midazolam and alprazolam metabolites. Microflow produced a 2-4x signal increase for the neat drug cocktail and a 5-10x increase for extracted plasma samples. Conclusion: The OptiFlow method consistently gave increased signal response relative to the IonDrive method and enabled a better lower limit of quantitation for defining phamacokinetics.