Rapid detection of Klebsiella pneumoniae using an auto-calibrated ISFET-array Lab-on-Chip platform

This paper presents the rapid detection of Klebsiella pneumonia, a major worldwide source and shuttle for antibiotic resistance, by a CMOS-based Lab-on-chip (LoC) platform. The LoC platform comprises a 64 × 200 ISFET array including a programmable gate for mismatch calibration. Calibration takes place on a pixel per pixel basis, with the array carrying-out simultaneous calibration and readout. The LoC platform is used to detect the pH changes occurring during DNA amplification and is demonstrated for the rapid detection of Klebsiella pneumoniae using isothermal molecular methods. Nucleic acids from the bacterial strain have been isolated from pure microbiological cultures and are detected in under 10 minutes. Overall, the presented Lab-on-Chip platform paired with the molecular methods hold significant potential for the rapid detection of Klebsiella pneumoniae at the point-of-care.