Molecular cloning and characterization of serine protease inhibitor from food-borne nematode, Gnathostoma spinigerum (vol 204, 105288, 2020)

Anusorn Tinyou,Salisa Chaimon,Orawan Phuphisut,Porntida Kobpornchai,Preeyarat Malaithong,Akkarin Poodeepiyasawat, Issariya Ieamsuwan,Jiraporn Ruangsittichai,Pornpan Pumirat,Paron Dekumyoy,Onrapak Reamtong,Poom Adisakwattana

ACTA TROPICA（2023）

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摘要

•3D simulation of GsSerp revealed 9 helices, 3 β-sheets, and a RCL.•rGsSerp was successfully expressed in E. coli.•Native GsSerp was detected in CWA but not in excretory–secretory products.•GsSerp was localized in the hemolymph and intestinal tissue.•rGsSerp could inhibit chymotrypsin.

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关键词

Gnathostoma spinigerum,Serine protease inhibitor,Serine protease,Recombinant protein

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